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HeLa [Chang Liver]人张氏肝细胞[STR鉴定正确]

产品型号:BH-C004
产品规格:1×10^6cell/T25瓶
产品价格:¥1320

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商品详情相关试剂说明书下载参考文献

商品详情

目录号

BH-C004

细胞英文(简称)

Chang liver

细胞名称

Chang liver 人张氏肝细胞【已通过STR鉴定】

背景资料

张氏肝细胞1954年从非恶性的人体组织中建立。广泛应用于病毒学、生化和恶性肿瘤相关的转移研究。ATCC通过同工酶分析、HeLA标记染色体和DNA指纹法分析,认为它的起源是HeLa细胞污染的。

细胞来源

ATCC

代次

P3

规格

冻存管/培养瓶

细胞数

1x10^6 cells

生物安全级别

1

组织来源

细胞形态

贴壁;上皮细胞样

细胞活力

95%(Viability by Trypan Blue Exclusion

细胞检测

细胞不含有HIV-1HBVHCV、支原体、细菌、酵母和真菌

培养条件

RPMI-1640 +10% FBS(货号:C2056-1A+1%P/S37℃5% CO2

传代方法

建议1:2-1:3 两天换液一次

冻存条件

无血清细胞冻存液(货号:S002

参考文献

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Human cells in continuous culture. I. Derivation of cell strains from esophagus, palate, liver, and lung.
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Cell Culture Collection Committee
Animal cell strains. The Cell Culture Collection Committee has assembled and certified 23 strains of animal cells.
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PubMed=5641128; DOI=10.1038/217750a0
Gartler S.M.
Apparent Hela cell contamination of human heteroploid cell lines.
Nature 217:750-751(1968)

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Chromosome abnormalities in human tumor cells in culture.
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HeLa cultures defined.
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Growth enhancing property of human monocytes from normal donors and cancer patients.
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A conspiracy of cells. One woman's immortal legacy and the medical scandal it caused.
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PubMed=18655026; DOI=10.1002/pmic.200700887
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Proteomic analysis of ubiquitinated proteins in normal hepatocyte cell line Chang liver cells.
Proteomics 8:2885-2896(2008)

PubMed=20143388; DOI=10.1002/ijc.25242
Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A., MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R., Freshney R.I.
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PubMed=21656536; DOI=10.1002/hep.24475
Gao Q., Wang X.-Y., Zhou J., Fan J.
Cell line misidentification: the case of the Chang liver cell line.
Hepatology 54:1894-1895(2011)

PubMed=23925245; DOI=10.1038/nature12064
Adey A., Burton J.N., Kitzman J.O., Hiatt J.B., Lewis A.P., Martin B.K., Qiu R., Lee C., Shendure J.
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Nature 500:207-211(2013)

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