商品详情

目录号	BH-C504
细胞英文(简称）	661W
细胞名称	661W小鼠视网膜感光细胞
背景资料	收到细胞请检查包装是否有破损，如有异常请尽快与我们联系!
细胞来源	国内
代次	P3
规格	冻存管/培养基
细胞数	1x10^6 cells
生物安全级别	1
组织来源	视网膜
细胞形态	贴壁；上皮样
细胞活力	95％（Viability by Trypan Blue Exclusion）
细胞检测	细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌
培养条件	F12K+10% FBS（货号：C2056-1A）+1%P/S；37℃，5% CO2
传代方法	建议1:2-1:3 两天换液一次
冻存条件	无血清细胞冻存液（货号：S002）

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参考文献

PubMed=23975727; DOI=10.1167/iovs.13-12085

Krishnamoorthy R.R., Clark A.F., Daudt D., Vishwanatha J.K., Yorio T.
A forensic path to RGC-5 cell line identification: lessons learned.
Invest. Ophthalmol. Vis. Sci. 54:5712-5719(2013)

PubMed=26854824; DOI=10.1016/j.exer.2016.01.016; PMCID=PMC5024725
Pfeffer B.A., Xu L.-B., Porter N.A., Rao S.R., Fliesler S.J.
Differential cytotoxic effects of 7-dehydrocholesterol-derived oxysterols on cultured retina-derived cells: dependence on sterol structure, cell type, and density.
Exp. Eye Res. 145:297-316(2016)

PubMed=29203899; DOI=10.1038/s41598-017-17241-0; PMCID=PMC5715133
Sayyad Z., Sirohi K., Radha V., Swarup G.
661W is a retinal ganglion precursor-like cell line in which glaucoma-associated optineurin mutants induce cell death selectively.
Sci. Rep. 7:16855-16855(2017)

PubMed=31024622; DOI=10.3389/fgene.2019.00308; PMCID=PMC6459963
Wheway G., Nazlamova L., Turner D., Cross S.
661W photoreceptor cell line as a cell model for studying retinal ciliopathies.
Front. Genet. 10:308.1-308.14(2019)

PubMed=31819341; PMCID=PMC6887793
Sanchez-Bretano A., Suen T.-C., Baba K., DeBruyne J.P., Tosini G.
Melatonin receptor heterodimerization in a photoreceptor-like cell line endogenously expressing melatonin receptors.
Mol. Vis. 25:791-799(2019)

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661W小鼠视网膜感光细胞

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