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C2C12小鼠成肌细胞

产品型号:BH-C406
产品规格:1x10^6cell/T25瓶
产品价格:¥1320

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商品详情相关试剂说明书下载参考文献

商品详情

目录号

BH-C406

细胞英文(简称)

C2C12

细胞名称

C2C12小鼠成肌细胞

背景资料

这是D.Yaffe O.Saxel建立的小鼠成肌细胞株的亚克隆(H.Blau等人构建)C2C12细胞株分化很快,形成可伸缩的肌管并生成特征性的肌蛋白。用骨形成蛋白2(BMP-2)处理,导致分化途径从成肌细胞转换成成骨细胞。检测表明肢骨发育畸形病毒(鼠痘)阴性。

细胞来源

ATCC CRL-1772

代次

P3

规格

冻存管/培养基

细胞数

1x10^6 cells

生物安全级别

1

组织来源

肌肉

细胞形态

贴壁;成肌细胞

细胞活力

95%(Viability by Trypan Blue Exclusion

细胞检测

细胞不含有HIV-1HBVHCV、支原体、细菌、酵母和真菌

培养条件

DMEM(高糖) +10% FBS(货号:C2056-1A+1%P/S37℃5% CO2

传代方法

建议1:2-1:3 两天换液一次

冻存条件

无血清细胞冻存液(货号:S002

参考文献

PubMed=563524; DOI=10.1038/270725a0
Yaffe D., Saxel O.
Serial passaging and differentiation of myogenic cells isolated from dystrophic mouse muscle.
Nature 270:725-727(1977)

PubMed=8023908; DOI=10.1152/ajpcell.1994.266.6.C1795
McMahon D.K., Anderson P.A.W., Nassar R., Bunting J.B., Saba Z., Oakeley A.E., Malouf N.N.
C2C12 cells: biophysical, biochemical, and immunocytochemical properties.
Am. J. Physiol. 266:C1795-C1802(1994)

PubMed=15596414
Burattini S., Ferri P., Battistelli M., Curci R., Luchetti F., Falcieri E.
C2C12 murine myoblasts as a model of skeletal muscle development: morpho-functional characterization.
Eur. J. Histochem. 48:223-233(2004)

PubMed=19298647; DOI=10.1186/1471-2121-10-20
Herrera B., Inman G.J.
A rapid and sensitive bioassay for the simultaneous measurement of multiple bone morphogenetic proteins. Identification and quantification of BMP4, BMP6 and BMP9 in bovine and human serum.
BMC Cell Biol. 10:20.1-20.11(2009)

PubMed=19350625; DOI=10.1002/bit.22318
Fujita H., Shimizu K., Nagamori E.
Novel method for fabrication of skeletal muscle construct from the C2C12 myoblast cell line using serum-free medium AIM-V.
Biotechnol. Bioeng. 103:1034-1041(2009)

PubMed=20635352; DOI=10.1002/bit.22865
Fujita H., Endo A., Shimizu K., Nagamori E.
Evaluation of serum-free differentiation conditions for C2C12 myoblast cells assessed as to active tension generation capability.
Biotechnol. Bioeng. 107:894-901(2010)

PubMed=24735950; DOI=10.1016/j.scr.2014.03.004
Willkomm L., Schubert S., Jung R., Elsen M., Borde J., Gehlert S., Suhr F., Bloch W.
Lactate regulates myogenesis in C2C12 myoblasts in vitro.
Stem Cell Res. 12:742-753(2014)

PubMed=25277546; DOI=10.1186/1471-2164-15-847
Didion J.P., Buus R.J., Naghashfar Z., Threadgill D.W., Morse H.C. III, Pardo-Manuel de Villena F.
SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy.
BMC Genomics 15:847.1-847.11(2014)

PubMed=25894527; DOI=10.1371/journal.pone.0121314
Bausch-Fluck D., Hofmann A., Bock T., Frei A.P., Cerciello F., Jacobs A., Moest H., Omasits U., Gundry R.L., Yoon C., Schiess R., Schmidt A., Mirkowska P., Hartlova A., Van Eyk J.E., Bourquin J.-P., Aebersold R., Boheler K.R., Zandstra P.W., Wollscheid B.
A mass spectrometric-derived cell surface protein atlas.
PLoS ONE 10:E0121314-E0121314(2015)

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