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P815小鼠肥大细胞瘤细胞

产品型号:BH-C442
产品规格:1x10^6cell/T25瓶
产品价格:¥1320

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商品详情相关试剂说明书下载参考文献

商品详情

目录号

BH-C442

细胞英文(简称)

P815

细胞名称

P815小鼠肥大细胞瘤细胞

背景资料

该细胞源自DBA/2小鼠的肥大细胞瘤;可吞噬乳胶微球,但不吞噬酵母聚糖或卡介苗,没有抗体依赖的细胞介导的细胞毒作用。硫酸右旋糖酐、LPSPPD不能抑制细胞生长;产生溶菌酶。

细胞来源

ATCC

代次

P3

规格

冻存管/培养基

细胞数

1x10^6 cells

生物安全级别

1

组织来源

肥大细胞;肥大细胞瘤

细胞形态

悬浮+贴壁;肥大细胞

细胞活力

95%(Viability by Trypan Blue Exclusion

细胞检测

细胞不含有HIV-1HBVHCV、支原体、细菌、酵母和真菌

培养条件

RPMI-1640 +10% FBS(货号:C2056-1A+1%P/S37℃5% CO2

传代方法

建议1:2-1:3 两天换液一次

冻存条件

无血清细胞冻存液(货号:S002

参考文献

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'Natural' killer cells in the mouse. I. Cytotoxic cells with specificity for mouse Moloney leukemia cells. Specificity and distribution according to genotype.
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Comparison of serotonin-producing murine mastocytomas, P-815 and FMA3: determination of tryptophan hydroxylase, aromatic L-amino acid decarboxylase, and cellular concentration of tryptophan, 5- hydroxytryprophan, 5-hydroxytryptamine and 5-hydroxyindoleacetic acid.
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Murine macrophage cell lines can be ordered in a linear differentiation sequence.
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Tsujimura T., Furitsu T., Morimoto M., Isozaki K., Nomura S., Matsuzawa Y., Kitamura Y., Kanakura Y.
Ligand-independent activation of c-kit receptor tyrosine kinase in a murine mastocytoma cell line P-815 generated by a point mutation.
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PubMed=10210327; DOI=10.1016/S0301-472X(98)00075-7
London C.A., Galli S.J., Yuuki T., Hu Z.-Q., Helfand S.C., Geissler E.N.
Spontaneous canine mast cell tumors express tandem duplications in the proto-oncogene c-kit.
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PubMed=18432777; DOI=10.1002/0471142735.im2004s43
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The P815 mastocytoma tumor model.
Curr. Protoc. Immunol. 43:20.4.1-20.4.18(2001)

PubMed=25277546; DOI=10.1186/1471-2164-15-847
Didion J.P., Buus R.J., Naghashfar Z., Threadgill D.W., Morse H.C. III, Pardo-Manuel de Villena F.
SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy.
BMC Genomics 15:847.1-847.11(2014)

PubMed=31220119; DOI=10.1371/journal.pone.0218412
Almeida J.L., Dakic A., Kindig K., Kone M., Letham D.L.D., Langdon S., Peat R., Holding-Pillai J., Hall E.M., Ladd M., Shaffer M.D., Berg H., Li J.-L., Wigger G., Lund S., Steffen C.R., Fransway B.B., Geraghty B., Natoli M., Bauer B., Gollin S.M., Lewis D.W., Reid Y.A.
Interlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell lines.
PLoS ONE 14:E0218412-E0218412(2019)

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