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DH82 狗肾恶性组织细胞增生症细胞

产品型号:BH-C722
产品规格:1x10^6cell / T25瓶
产品价格:¥1320

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商品详情相关试剂说明书下载参考文献

商品详情

目录号

BH-C722

细胞英文(简称)

DH82

细胞名称

DH82狗肾恶性组织细胞增生症细胞

背景资料

 源自一只患有恶性组织细胞增生症的十岁雄性金毛猎犬。细胞具有巨噬细胞样形态,能够吞噬乳胶颗粒。它们对 Fc-γ 受体呈阳性,对 Fc-mu C3b 受体呈阴性。DH82 细胞不产生 IL-1

细胞来源

ATCC

代次

P3

规格

冻存管/培养瓶

细胞数

1x10^6 cells

生物安全级别

1

组织来源

细胞形态

贴壁;上皮细胞样

细胞活力

95%(Viability by Trypan Blue Exclusion

细胞检测

细胞不含有HIV-1HBVHCV、支原体、细菌、酵母和真菌

培养条件

DMEM(高糖)+10%FBS+1% P/S

传代方法

建议1:2-1:3 两天换液一次

冻存条件

无血清细胞冻存液(货号:S002

参考文献

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A macrophage-monocyte cell line from a dog with malignant histiocytosis.
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Growing Ehrlichia species in a continuous cell line.
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PubMed=11585095
Zweygarth E., Josemans A.I.
Continuous in vitro propagation of Cowdria ruminantium (Welgevonden stock) in a canine macrophage-monocyte cell line.
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The Kumm isolate of Ehrlichia ruminantium: in vitro isolation, propagation and characterization.
Onderstepoort J. Vet. Res. 69:147-153(2002)

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Polymerase chain reaction-based species verification and microsatellite analysis for canine cell line validation.
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Heinrich F., Contioso V.B., Stein V.M., Carlson R., Tipold A., Ulrich R., Puff C., Baumgartner W., Spitzbarth I.
Passage-dependent morphological and phenotypical changes of a canine histiocytic sarcoma cell line (DH82 cells).
Vet. Immunol. Immunopathol. 163:86-92(2015)

PubMed=27257868; DOI=10.1371/journal.pone.0156689
Maeda J., Froning C.E., Brents C.A., Rose B.J., Thamm D.H., Kato T.A.
Intrinsic radiosensitivity and cellular characterization of 27 canine cancer cell lines.
PLoS ONE 11:E0156689-E0156689(2016)

PubMed=26842912; DOI=10.1111/vco.12197
Hartley G., Faulhaber E., Caldwell A., Coy J., Kurihara J., Guth A., Regan D., Dow S.
Immune regulation of canine tumour and macrophage PD-L1 expression.
Vet. Comp. Oncol. 15:534-549(2017)

PubMed=27197945; DOI=10.1111/vco.12192
Fowles J.S., Dailey D.D., Gustafson D.L., Thamm D.H., Duval D.L.
The Flint Animal Cancer Center (FACC) canine tumour cell line panel: a resource for veterinary drug discovery, comparative oncology and translational medicine.
Vet. Comp. Oncol. 15:481-492(2017)

PubMed=28620374; DOI=10.3389/fimmu.2017.00604
Mendonca P.H.B., da Rocha R.F.D.B., de Braz Moraes J.B., LaRocque-de-Freitas I.F., Logullo J., Morrot A., Nunes M.P., Freire-de-Lima C.G., Decote-Ricardo D.
Canine macrophage DH82 cell line as a model to study susceptibility to Trypanosoma cruzi infection.
Front. Immunol. 8:604.1-604.11(2017)

PubMed=31175136; DOI=10.1158/1535-7163.MCT-18-1346
Das S., Idate R., Cronise K.E., Gustafson D.L., Duval D.L.
Identifying candidate druggable targets in canine cancer cell lines using whole exome sequencing.
Mol. Cancer Ther. 18:1460-1471(2019)

PubMed=33389257; DOI=10.1007/s10096-020-04106-0
Wurtz N., Penant G., Jardot P., Duclos N., La Scola B.
Culture of SARS-CoV-2 in a panel of laboratory cell lines, permissivity, and differences in growth profile.
Eur. J. Clin. Microbiol. Infect. Dis. 40:477-484(2021)

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